Sabiia Seb
PortuguêsEspañolEnglish
Embrapa
        Busca avançada

Botão Atualizar


Botão Atualizar

Ordenar por: 

RelevânciaAutorTítuloAnoImprime registros no formato resumido
Registros recuperados: 27
Primeira ... 12 ... Última
Imagem não selecionada

Imprime registro no formato completo
Acoustic and optical variations during rapid downward motion episodes in the deep north-western Mediterranean Sea ArchiMer
Van Haren, H.; Taupier-letage, I.; Aguilar, J. A.; Albert, A.; Anghinolfi, M.; Anton, G.; Anvar, S.; Ardid, M.; Assis Jesus, A. C.; Astraatmadja, T.; Aubert, J. -j.; Auer, R.; Baret, B.; Basa, S.; Bazzotti, M.; Bertin, V.; Biagi, S.; Bigongiari, C.; Bou-cabo, M.; Bouwhuis, M. C.; Brown, A.; Brunner, J.; Busto, J.; Camarena, F.; Capone, A.; Carminati, G.; Carr, J.; Castel, D.; Castorina, E.; Cavasinni, V.; Cecchini, S.; Charvis, Ph.; Chiarusi, T.; Circella, M.; Coniglione, R.; Costantini, H.; Cottini, N.; Coyle, P.; Curtil, C.; De Bonis, G.; Decowski, M. P.; Dekeyser, I.; Deschamps, Anne; Distefano, C.; Donzaud, C.; Dornic, D.; Drouhin, D.; Eberl, T.; Emanuele, U.; Ernenwein, J. -p.; Escoffier, S.; Fehr, F.; Flaminio, V.; Fratini, K.; Fritsch, U.; Fuda, J. -l.; Giacomelli, G.; Gomez-gonzalez, J. P.; Graf, K.; Guillard, G.; Halladjian, G.; Hallewell, G.; Heijboer, A. J.; Hello, Y.; Hernandez-rey, J. J.; Hoessl, J.; De Jong, M.; Kalantar-nayestanaki, N.; Kalekin, O.; Kappes, A.; Katz, U.; Kooijman, P.; Kopper, C.; Kouchner, A.; Kretschmer, W.; Lahmann, R.; Lamare, P.; Lambard, G.; Larosa, G.; Laschinsky, H.; Lefevre, D.; Lelaizant, G.; Lim, G.; Lo Presti, D.; Loehner, H.; Loucatos, S.; Lucarelli, F.; Lyons, K.; Mangano, S.; Marcelin, M.; Margiotta, A.; Martinez-mora, J. A.; Maurin, G.; Mazure, A.; Melissas, M.; Montaruli, T.; Morganti, M.; Moscoso, L.; Motz, H.; Naumann, C.; Neff, M.; Ostasch, R.; Palioselitis, G.; Pavalas, G. E.; Payre, P.; Petrovic, J.; Piattelli, P.; Picot-clemente, N.; Picq, C.; Pillet, R.; Popa, V.; Pradier, T.; Presani, E.; Racca, C.; Radu, A.; Reed, C.; Riccobene, G.; Richardt, C.; Rujoiu, M.; Russo, G. V.; Salesa, F.; Schoeck, F.; Schuller, J. -p.; Shanidze, R.; Simeone, F.; Spurio, M.; Steijger, J. J. M.; Stolarczyk, Th.; Tamburini, C.; Tasca, L.; Toscano, S.; Vallage, B.; Van Elewyck, V.; Vecchi, M.; Vernin, P.; Wijnker, G.; De Wolf, E.; Yepes, H.; Zaborov, D.; Zornoza, J. D.; Zuniga, J..
An Acoustic Doppler Current Profiler (ADCP) was moored at the deep-sea site of the ANTARES neutrino telescope near Toulon, France, thus providing a unique opportunity to compare high-resolution acoustic and optical observations between 70 and 170 m above the sea bed at 2475 m. The ADCP measured downward vertical currents of magnitudes up to 0.03 m s(-1) in late winter and early spring 2006. In the same period, observations were made of enhanced levels of acoustic reflection, interpreted as suspended particles including zooplankton, by a factor of about 10 and of horizontal currents reaching 0.35 m s(-1). These observations coincided with high light levels detected by the telescope, interpreted as increased bioluminescence. During winter 2006 deep...
Tipo: Text Palavras-chave: Acoustic ADCP observations; Optical photo-multiplier observations; Deep Mediteranean; ANTARES neutrino telescope; Episodic downward current; Bioluminescence; Dense water formation; Northern boundary current.
Ano: 2011 URL: https://archimer.ifremer.fr/doc/00303/41412/40598.pdf
Imagem não selecionada

Imprime registro no formato completo
Bioluminescent bacteria: lux genes as environmental biosensors BJM
Nunes-Halldorson,Vânia da Silva; Duran,Norma Letícia.
Bioluminescent bacteria are widespread in natural environments. Over the years, many researchers have been studying the physiology, biochemistry and genetic control of bacterial bioluminescence. These discoveries have revolutionized the area of Environmental Microbiology through the use of luminescent genes as biosensors for environmental studies. This paper will review the chronology of scientific discoveries on bacterial bioluminescence and the current applications of bioluminescence in environmental studies, with special emphasis on the Microtox toxicity bioassay. Also, the general ecological significance of bioluminescence will be addressed.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacteria; Bioluminescence; Lux genes; Microtox toxicity bioassay.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000200001
Imagem não selecionada

Imprime registro no formato completo
Bioluminescent Coleoptera of Biological Station of Boracéia (Salesópolis, SP, Brazil): diversity, bioluminescence and habitat distribution Biota Neotropica
Viviani,Vadim Ravara; Santos,Raphael Machado dos.
Brazil hosts the richest biodiversity of bioluminescent beetles in the world. Several species are found in the Atlantic rain forest, one of the richest and most threatened tropical forests in the world. We have catalogued the biodiversity of bioluminescent species mainly of Elateroidea superfamily occurring in one of the last largest and most preserved remnants of Atlantic rain forest, located at the Biological Station of Boracéia of São Paulo University (Salesopolis, SP, Brazil). This site accounted with the largest diversity ever found for a single place in São Paulo State, with 39 species: Lampyridae (30), Phengodidae (5), Elateridae (3) and Staphylinidae (1). This fauna has unique species that were not found in any other places of Atlantic forest in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lampyridae; Phengodidae; Elateridae; Staphylinidae; Bioluminescence; Fireflies; Bioindicators.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032012000300001
Imagem não selecionada

Imprime registro no formato completo
Brazilian Bioluminescent Beetles: Reflections on Catching Glimpses of Light in the Atlantic Forest and Cerrado Anais da ABC (AABC)
BECHARA,ETELVINO J.H.; STEVANI,CASSIUS V..
ABSTRACT Bioluminescence - visible and cold light emission by living organisms - is a worldwide phenomenon, reported in terrestrial and marine environments since ancient times. Light emission from microorganisms, fungi, plants and animals may have arisen as an evolutionary response against oxygen toxicity and was appropriated for sexual attraction, predation, aposematism, and camouflage. Light emission results from the oxidation of a substrate, luciferin, by molecular oxygen, catalyzed by a luciferase, producing oxyluciferin in the excited singlet state, which decays to the ground state by fluorescence emission. Brazilian Atlantic forests and Cerrados are rich in luminescent beetles, which produce the same luciferin but slightly mutated luciferases, which...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coleoptera; Bioluminescence; Luciferase; Luciferin; Luminous termitary; Oxidative stress..
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000200663
Imagem não selecionada

Imprime registro no formato completo
Climate impact on long-term changeability of the black sea bioluminescence field and plankton community characteristics. IBSS Repository
Tokarev, Yu.; Mel'nikov, V.; Burmistrova, N.; Belokopytov, V.; Temnykh, A..
Researches in the bioluminescence field vertical structure, amplitude characteristics and spatial distribution in the Black Sea have been conducted in IBSS, NAS of Ukraine, from up to the present time. Enough evidencies are available to testify dramatic changes in different Black sea ecosystem components including bioluminescence field parameters, which took place last decades. But still there is no direct answer to the question about the reasons for such transformations, as variability of the Black Sea biophysical (bioluminescence) and biological characteristics at the climatic time scales, i.e. at the scale of decade periods are not sufficiently studied now. The most pronounced changes in vertical structure of the bioluminescence field are observed in...
Tipo: Proceedings Paper Palavras-chave: Climate changeability; Cold intermediate layer; Bioluminescence; Plankton.
Ano: 2008 URL: http://repository.ibss.org.ua/dspace/handle/99011/694
Imagem não selecionada

Imprime registro no formato completo
Description of Coctilelater minimus sp. nov. (Coleoptera, Elateridae, Agrypninae) Rev. Bras. entomol.
Rosa,Simone Policena.
Coctilelater minimus from Brazil (Pará) is described and illustrated. This new species is mainly characterized by small size and coloration pattern.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amazonian Forest; Bioluminescence; Brazil; Click beetles; Neotropical Region.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262009000100009
Imagem não selecionada

Imprime registro no formato completo
Description of the larva of Alampoides alychnus (Kirsch, 1873), the first known species with bioluminescent immatures in Euplinthini (Elateridae, Agrypninae) Pap. Avulsos de Zool. (São Paulo)
Rosa,Simone Policena; Costa,Cleide.
Mature larva of Alampoides alychnus (Kirsch) is described and compared to known Pyrophorini immatures. Larvae were collected live in the soil of a region dominated by sugarcane plantation and gallery forest in Campo Novo dos Parecis, Mato Grosso, Brazil. They were maintained in laboratory and the pupal period lasted 14 days. This larva differs from other Pyrophorini larvae mainly by bioluminescent pattern: one pair of luminous spots on the mesonotum, and a longitudinal series of median spots on the metanotum and all abdominal segments. The morphology of larva and the bioluminescent pattern of larva and pupa are described for the first time to the genus and the tribe. The fact that adults show no trace of luminescence is emphasized.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coleoptera; Immatures; Bioluminescence; Click Beetles; Firefly; Neotropical Region.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0031-10492013002200001
Imagem não selecionada

Imprime registro no formato completo
Détection de la coelentérazine chez cinq espèces de Myctophidae du plateau des Iles Kerguelen ArchiMer
Duchatelet, Laurent; Hermans, Claire; Duhamel, Guy; Cherel, Yves; Guinet, Christophe; Mallefet, Jérôme.
Myctophidae comprise the largest biomass of mesopelagic fishes in the Southern Ocean. This family has the ability to emit light – bioluminescence – via specific organs called photophores, and from the head and caudal organs. Through biochemical analyses, this study reveals that coelenterazine is the substrate for light reaction in the five most common myctophid species from the Southern Ocean. The results also support the hypothesis of the acquisition of this molecule via the food chain and reveal the presence of two stabilised storage forms of coelenterazine (enol-sulfate and dehydrocoelenterazine) within various tissues of the studied species. Finally, detection of coelenterazine in the gonads also suggests the potential maternal transfer of the...
Tipo: Text Palavras-chave: Myctophidae; Bioluminescence; Coelenterazine; Dehydrocoelenterazine; Enol-sulfate; Biochemical assays.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00502/61405/65084.pdf
Imagem não selecionada

Imprime registro no formato completo
Ecophysiological studies on luminous bacteria associated with marine gastropods. ArchiMer
Ramesh, A; Venugopalan, V.
Information available on the association of luminous bacteria with marine invertebrates is limited. Hence qualitative and quantitative investigations were undertaken with a view to understand the distribution of luminous microflora in relation to the environmental factors and also in the gut of two marine gastropods, Aplysia benedicti Eliot and Bullia tranquebarica (Roding) from Porto Novo (11 degree 29'N, 79 degree 46'E) waters of the East coast of India. The gut of the gastropods harboured maximum number of luminous bacteria when compared to the water and sediment. Qualitative analysis revealed the presence of two luminous bacterial components, viz. Vibrio harveyi and V. fishceri. V. fischeri was not found in association with A. benedicti . The isolates...
Tipo: Text Palavras-chave: Gastropoda; Vibrio fischeri; Vibrio harveyi; Bioluminescence; Bacteria; Physicochemical properties; Environmental factors; Microorganisms; Luminous organisms; Interspecific relationships; Ecophysiology.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-993.pdf
Imagem não selecionada

Imprime registro no formato completo
Éco-régionalisation et conservation des communautés benthiques de la zone économique exclusive française des îles Kerguelen ArchiMer
Martin, Alexis; Trouslard, Emmanuelle; Hautecoeur, Mélyne; Blettery, Jonathan; Moreau, Camille; Saucède, Thomas; Améziane, Nadia; Duhamel, Guy; Eléaume, Marc.
The deep-sea benthic ecosystems of the French Kerguelen exclusive economic zone remain poorly understood. To address benthic conservation issues, the authors recently contributed expert knowledge to guide the recent extension of the Marine Reserve of the ‘Terres australes françaises’. In this new study, we propose a benthic ecoregionalisation of the northern Kerguelen Plateau based on a measurable and repeatable methodology that relies on the generalised dissimilarity modelling technique. Data of macro-epibenthic invertebrate species from the POKER 2 (2010) fish stock assessment survey have been used to compute the models. Our results allow us (i) to characterise and map benthic landscape units based on assemblages of marine invertebrates pooled by taxa...
Tipo: Text Palavras-chave: Myctophidae; Bioluminescence; Coelenterazine; Dehydrocoelenterazine; Enol-sulfate; Biochemical assays.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00505/61671/65598.pdf
Imagem não selecionada

Imprime registro no formato completo
Evaluation of meso-substituted cationic corroles as potential antibacterial agents Anais da ABC (AABC)
CARDOTE,TERESA A.F.; BARATA,JOANA F.B.; AMADOR,CAROLINA; ALVES,ELIANA; NEVES,MARIA GRAÇA P.M.S.; CAVALEIRO,JOSÉ A.S.; CUNHA,ÂNGELA; ALMEIDA,ADELAIDE; FAUSTINO,MARIA AMPARO F..
ABSTRACT Cationic derivatives of 5,10,15-tris[4-(pyridin-4-ylsulphanyl)-2,3,5,6-tetrafluorophenyl]-corrolategallium(III)pyridine and 5,10,15-tris[4-(pyridin-2-ylsulfanyl)-2,3,5,6-tetrafluorophenyl]-correlategallium(III)pyridine were synthesized and their photosensitizing properties against the naturally bioluminescent Gram-negative bacterium Allivibrio fischeri were evaluated. The cationic corrole derivatives exhibited antibacterial activity at micromolar concentrations against this Gram-negative bacterium strain.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allivibrio fischeri; Corroles; Photosensitizer; PDI; Bioluminescence.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000301175
Imagem não selecionada

Imprime registro no formato completo
High-Throughput Identification of Candidate Strains for Biopreservation by Using Bioluminescent Listeria monocytogenes ArchiMer
El Kheir, Sara M.; Cherrat, Lamia; Awussi, Ahoefa A.; Ramia, Nancy E.; Taha, Samir; Rahman, Abdur; Passerini, Delphine; Leroi, Francoise; Petit, Jeremy; Mangavel, Cecile; Revol-junelles, Anne-marie; Borges, Frederic.
This article describes a method for high-throughput competition assays using a bioluminescent strain of L. monocytogenes. This method is based on the use of the luminescent indicator strain L. monocytogenes EGDelux. The luminescence of this strain is correlated to growth, which make it suitable to monitor the growth of L. monocytogenes in mixed cultures. To this aim, luminescence kinetics were converted into a single numerical value, called the Luminescence Disturbance Indicator (LDI), which takes into account growth inhibition phenomena resulting in latency increase, decrease in the luminescence rate, or reduction of the maximum luminescence. The LDI allows to automatically and simultaneously handle multiple competition assays which are required for...
Tipo: Text Palavras-chave: Biopreservation; Listeria monocytogenes; High-throughput screening assays; Bioluminescence; Competition; Mixed culture; Bioprotection; Antibacterial activities.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00454/56534/58236.pdf
Imagem não selecionada

Imprime registro no formato completo
Hormesis response of marine and freshwater luminescent bacteria to metal exposure Biol. Res.
SHEN,KAILI; SHEN,CHAOFENG; LU,YUAN; TANG,XIANJIN; ZHANG,CONGKAI; CHEN,XINCAI; SHI,JIYAN; LIN,QI; CHEN,YINGXU.
The stimulatory effect of low concentrations of toxic chemicals on organismal metabolism, referred to as hormesis, has been found to be common in the widely used luminescence bioassay. This paper aims to study the hormesis phenomenon in both marine and freshwater luminescent bacteria, named Photobacterium phosphorem and Vibrio qinghaiensis. The effects of Cu (II), Zn (II), Cd (II) and Cr (VI) on luminescence of these two bacteria were studied for 0 to 75 minutes exposure by establishing dose- and time-response curves. A clear hormesis phenomenon was observed in all four testing metals at low concentrations under the condition of luminescence assays.
Tipo: Journal article Palavras-chave: Hormesis; Bioluminescence; Q67; T3.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000200006
Imagem não selecionada

Imprime registro no formato completo
Influence of seamounts at the Atlantic Ocean on modification of the bioluminiscence and plankton characteristics IBSS Repository
Tokarev, Yu. N.; Bityukov, E. P.; Williams, R..
The bioluminescence and plankton characteristics in the euphotic layer over 11 seamounts in the Atlantic Ocean were studied. Investigations were carried out along the axis of flow over the mounts. Bathyphotometric measurements (1606 casts) were taken from 2 m to 100 m depths at 87 stations over the various seamounts with a vertical resolution of 1 m. Plankton samples were taken with Juday nets and submersible electric pumps with 36 m3• •h-1 performance. The bioluminescence characteristics varied over seamounts with different geomorphological features. The hydrodynamic gyres, created by the bottom elevations, and the upwelling of water with biogenic elements, also seem to have effects on the quantitative characteristics of the plankton. The largest changes...
Tipo: Journal Contribution Palavras-chave: Atlantic Ocean; Seamounts; Bioluminescence; Plankton; Атлантический океан; Подводные возвышенности; Биолюминесценция; Планктон Seamounts Bioluminescence Plankton Plankton http://aims.fao.org/aos/agrovoc/c_5950.
Ano: 2003 URL: http://repository.ibss.org.ua/dspace/handle/99011/168
Imagem não selecionada

Imprime registro no formato completo
Metapyrophorus pharolim a new genus and species of Pyrophorini (Coleoptera, Elateridae, Agrypninae) Rev. Bras. entomol.
Rosa,Simone Policena; Costa,Cleide.
Metapyrophorus, a new monotypic genus, is erected based on M. pharolim, new species from Trinidad and Tobago and Venezuela. The genus is characterized mainly by its pair of convex pronotal bioluminescent organs, equidistant between the median line and the lateral margin.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bioluminescence; Click-beetles; Neotropical Region; Systematic.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262009000100012
Imagem não selecionada

Imprime registro no formato completo
New data on the natural history and description of the immatures of Fulgeochlizus bruchi, a bioluminescent beetle from Central Brazil (Elateridae, Pyrophorini) Pap. Avulsos de Zool. (São Paulo)
Rosa,Simone Policena; Costa,Cleide; Higashi,Neide.
The mature larva and pupa of Fulgeochlizus bruchi (Candèze, 1896) are described and illustrated. Bioluminescent patterns are also given. Comments, new data on the first instar larva and natural history data are presented. The first instar larvae differ from the mature larvae mainly in their chaetotaxy, which is sparse and more symmetrically distributed.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agrypninae; Bioluminescence; Larva; Morphology; Neotropical Region; Pupa.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0031-10492010004100001
Imagem não selecionada

Imprime registro no formato completo
New species of Ignelater Costa (Coleoptera, Elateridae, Pyrophorini) Pap. Avulsos de Zool. (São Paulo)
Rosa,Simone Policena.
Ignelater inaguensis sp. nov. from Bahamas, Great Inagua Island is described, illustrated and compared to other Ignelater species. This species is characterized mainly by its elytra, slightly widened from humerus up to the middle or posterior third and the length ratio between elytra and pronotum 2.51-2.71.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agrypninae; Bioluminescence; Click beetles; Firefly; Neotropical region.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0031-10492010002900001
Imagem não selecionada

Imprime registro no formato completo
Plankton communities of the South Atlantic anticyclonic gyre ArchiMer
Piontkovski, Sa; Landry, Mr; Finenko, Zz; Kovalev, Av; Williams, R; Gallienne, Cp; Mishonov, Av; Skryabin, Va; Tokarev, Yn; Nikolsy, Vn.
Data collected during cruises of the Former Soviet Union (in 1963-1989) and the British Atlantic meridional transect program (in 1995-1999) were used to analyse macroscale patterns in phyto- and zooplankton biomass, size structure, species diversity, chlorophyll a, and plankton bioluminescence in the macroscale anticyclonic gyre of the South Atlantic Ocean. The spatial pattern of bioluminescence intensity was in good agreement with that of remotely sensed (CZCS) chlorophyll a, phosphate, salinity, and copepod species diversity index distributions especially in terms of geographic inclinations of the isolines, both associated with the north-westward pattern off the South equatorial current. Among the 416 copepod species recorded in samples, 51 species were...
Tipo: Text Palavras-chave: Zooplancton; Chlorophylle; Bioluminescence; Altantique Sud; Zooplankton; Chlorophyll; Bioluminescence; South Atlantic Ocean.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/00322/43271/43002.pdf
Imagem não selecionada

Imprime registro no formato completo
Quorum sensing in Enterococcus faecium, Enterococcus faecalis and Bacillus cereus strains isolated from ricotta processing Ciência Rural
Fernandes,Meg da Silva; Esper,Luciana Maria Ramires; Kabuki,Dirce Yorika; Kuaye,Arnaldo Yoshiteru.
ABSTRACT: The quorum sensing phenomenon is a process of intra- and inter-species microbial communication involving the production and detection of extracellular signaling molecules. The autoinducer AI-2 has been proposed to serve as a ‘universal signal’ for interspecies communication. This study aimed to evaluate the capability of Enterococcus faecium, Enterococcus faecalis, and Bacillus cereus strains isolated from ricotta processing to produce quorum sensing signalling molecules (AI-2). The strains were evaluated for the presence of the luxS gene using the polymerase chain reaction. AI-2 quorum sensing signalling molecules were measured in relative light units (RLUs) using a luminometer. A total of 74% of E. faecium, 91% of E. faecalis, and 95% of B....
Tipo: Info:eu-repo/semantics/article Palavras-chave: LuxS; AI-2; Vibrio harveyi; Bioluminescence; Enterococcus spp.; Bacillus cereus.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000200751
Imagem não selecionada

Imprime registro no formato completo
Simplified Transformation of Ostreococcus tauri Using Polyethylene Glycol ArchiMer
Sanchez, Frédéric; Geffroy, Solene; Norest, Manon; Yau, Sheree; Moreau, Hervé; Grimsley, Nigel.
Ostreococcus tauri is an easily cultured representative of unicellular algae (class Mamiellophyceae) that abound in oceans worldwide. Eight complete 13–22 Mb genomes of phylogenetically divergent species within this class are available, and their DNA sequences are nearly always present in metagenomic data produced from marine samples. Here we describe a simplified and robust transformation protocol for the smallest of these algae (O. tauri). Polyethylene glycol (PEG) treatment was much more efficient than the previously described electroporation protocol. Short (2 min or less) incubation times in PEG gave >104 transformants per microgram DNA. The time of cell recovery after transformation could be reduced to a few hours, permitting the experiment to be...
Tipo: Text Palavras-chave: Chlorophyta; Prasinophyte; Mamiellophyceae; Ostreococcus; Plankton; Picoeukaryote; Ecosystem; Bioluminescence; Promoter; Luciferase; Gene expression.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00500/61170/64639.pdf
Registros recuperados: 27
Primeira ... 12 ... Última
 

Empresa Brasileira de Pesquisa Agropecuária - Embrapa
Todos os direitos reservados, conforme Lei n° 9.610
Política de Privacidade
Área restrita

Embrapa
Parque Estação Biológica - PqEB s/n°
Brasília, DF - Brasil - CEP 70770-901
Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco

Valid HTML 4.01 Transitional